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Under anaerobic conditions only two mutants, which do not assimilate nitrate, were resistant against chlorate.
Home, bacteriology, nitrate Reduction Test: Principle, Procedure and Results.However, if there is still no color change after the addition of zinc, this indicates the nitrates were reduced beyond nitrites to ammonia or molecular nitrogen, and the organism is positive for nitrate reduction.Untersuchungen an intakten Zellen.No color change indicates the absence of nitrite.(Praha) 5, 165170 (1960).PubMed, google Scholar, cole,.Google Scholar, fujita,.: Studies on soluble cytochromes.(Tokyo) 60, 204215 (1966).When sulfanilic acid is added, it will react with the nitrous acid to produce diazotized sulfanilic acid. .Google Scholar Stouthamer,.PubMed Google Scholar : Mutant strains oncfs concours 2017 of Aerobacter aerogenes which require both methionine and lysine for aerobic growth.
Skip to main content, springerLink, article 38 Downloads 12 Citations, summary.
This can happen either because nitrate was not reduced or because nitrate was reduced to nitrite, then nitrite was further reduced to some other molecule.
Gest: A new procedure for assay of bacterial hydrogenases.
Becher: Die Reduktion von Perchlorat durch Bakterien.
Acta (Amst.) 82, 441444 (1964).
Evans: Preparation and some properties of a soluble nitrate reductase from Rhizobium japonicum.
Pichinoty: Alteration of respiratory particles by mutation.Acta (Amst.) 85, 377389 (1964).If no red color forms, there was no nitrate to reduce.Le Minor: Mutations affectant la nitrate réductase A et d'autres enzymes bactériennes d'oxydoréduction.Acta (Amst.) 128, 419425 (1966).Google Scholar Johnson,.After addition of Reagent A and Reagent B: Tube #1 is positive for nitrate reduction. .If the medium does not turn red after the addition of the reagents, it can mean that the organism was unable to reduce the nitrate, or the organism was able to denitrify the nitrate or nitrite to produce ammonia or molecular nitrogen.Keywords, nitrate Assimilation Growth Medium Anaerobic Condition Growth Temperature, these keywords were added by machine and not by the authors.Ranlett: A soluble c-type cytochrome from anaerobically grown Escherichia coli and various Enterobacteriaceae.